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The accuracy of this measurement is not affected by the total mass of protein loaded into each lane, nor by the length of staining of each gel, nor by the intensity by which the different substrates take up the Coomassie dye nor by the fluorescence of the particular protein-dye complex (the property being detected by the LiCor Odyssey scanner).
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Image analysis of each cell was performed to quantify the length of the comet and the intensity of staining.
To quantify the difference between wild-type and mutant axonemes, we measured the length of acetylated tubulin staining in equivalent retinal sections from multiple wild-type and mutant embryos at 7 d.p.f.
The average length of acetylated tubulin staining was 1.93 ± 0.87 µm in cc2d2a−/− photoreceptors compared with 3.8 ± 0.93 µm in cc2d2a+/+ or +/− photoreceptors (P < 0.0001, Student's t-test, n = 180 photoreceptors from 16 eyes).
First, we examined the effect of time to fixation and length of fixation on IHC staining for HER2 in NCI-N87, SCH, and SNU-16 tumor tissues.
The DNA content is determined by the intensity of the SYTOX Green stain and the nuclear morphology by the aspect ratio (ratio of width to length) of the DNA stain.
Briefly, Jurkat cells that were exposed to plant extracts in the presence or absence of HIV-1 infection for various lengths of time were stained in 0.2% trypan blue dye and then counted for viable cells under a light microscope.
Sections were stained throughout the length of vein exposed to Ad49GFP, and positive GFP staining was detected in ECs throughout the vessel.
Seven micron thick coronal sections were cut along the length of the spinal cord and stained with luxol fast blue.
The plates were incubated at 37 °C for the length of drug exposure, and then stained with methylene blue.
To evaluate the length of detached cilia in cytospin preparations, staining for ∃4 tubulin was performed on cytospin samples from 13 nonsmokers and 14 smokers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com