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However, the length of protrusion II was reduced and protrusion III appeared slimmer.
Given a measured length of protrusion L p, the radius of the spherical cap Rcap can be obtained (see Additional file 1).
The simulated cell retraction from the micropipette is measured in the reduction of length of protrusion (Fig. 3G), matching the retraction behavior seen in live cells.
The starting position of a protrusion on the membrane in frame n is set by the intersection of S n +1 and the boundary of I n, and the length of protrusion between frames n and (n +1) is given by the length of branch residing in (L1 in Figure 4D1).
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We found that expression of EphB2 resulted in a significant decrease in the average length of dendritic protrusions, while EphB2DN resulted in a significant increase in the length of protrusions (control, 2.22±0.06 µm; EphB2, 1.91±0.04 µm; EphB2DN, 2.49±0.08 µm; p<0.005, Figure 2F).
By analysis of Dami cells before and 2 min after LPA stimulation, we found that the cell diameter was reduced from 12.64 ± 1.32 to 11.4 ± 1.93 µm (P = 0.019), and the length of protrusions was 10 ± 3 µm.
Decide what overall desired length of protrusions at each end of the flapper tube you will need, and grind down any excess rod.
Changes in the length of dendritic protrusion would suggest that EphBs might alter the maturation or structure of dendritic protrusions rather than their overall number.
Protrusion rates were determined by using ImageJ Kymograph software (National Institutes of Health) and calculated as the length of the protrusion divided by the total time of the movie.
Closer examination of these protrusions, which occurred in approximately 30% of the images taken from G1 arrested samples, showed that when the length of the protrusion was taken into account, the diameter of the minichromosomes calculated from circumference measurements increases from 51.3 to 62.4nm, in keeping with the diameter measurements from samples that did not exhibit protrusions.
We expressed GFP-WT fascin 2b fusion protein in COS-7 cells and observed the formation of long filopodia (mean length ± standard error of the mean (SEM) = 6.04±0.21 µm; number of filopodia, Nfilopodia = 130; number of cells for which filopodial lengths were measured, Ncells = 26) (Figure 3A,E,I) with even distribution of GFP along the length of each protrusion.
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