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We also predicted (2) that effects of orientation would be less when the length of attached stolon was greater.
Dong et al. (2010) recently showed that the survival and growth of ramets of Alternanthera philoxeroides increased with increasing length of attached stolons.
We therefore hypothesized 1) that horizontal orientation can increase the survival and growth of small clonal fragments, and 2) that this effect of orientation will be less in fragments with greater length of attached stolons.
In this study, we build upon that previous finding to ask whether the orientation of dispersed, single-node clonal fragments can also affect their survival and growth, and whether this depends upon the length of attached stolon internodes, through their function as storage organs.
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Horizontal orientation of fragments from the horizontal stems of clonal plants might thus increase establishment of the fragments and this effect might be stronger in fragments with shorter lengths of attached stolon internodes and thus less stored resources.
The reactions with 5'-labeled bottom strand show that the cleaved bottom strand can be used as a primer for reverse transcription of the linear intron, yielding higher molecular weight product with different lengths of attached cDNA (Fig. 4C, lanes 1 and 2).
This elution order paralleled the increases in hydrophobicity of each lipid A species based on the total number and lengths of attached acyl chains and decreases in polarity based on the number of phosphate groups attached to the glucosamine groups.
Studies using proteinase K to cleave the VPg protein on picornavirus RNA, while leaving the tyrosyl-RNA phosphodiester bond intact, indicate that the length of the attached VPg is not important for unlinkase recognition [18], [19].
Midway up the first windward leg, with Prada's crew regaining its composure after a harsh lesson in starting-line tactics by the clinical Kiwis, the Italians' charge came to a crashing halt when they hooked a big piece of plastic, with a long length of rope attached, to the blunt, leading edge of their keel bulb some 12 feet below the waterline.
Micellar size was the only characteristic being affected by the length of the attached fatty acid.
But with 200 sprites packed into a space just 10cm by 20cm, each with a tightly coiled length of wire attached, whoever packs them up is going to have a tough job on their hands.
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