Sentence examples for length of amplification from inspiring English sources

For verification of the correct length of amplification products, they were analysed on an ethidium bromide stained 2% agarose gel.

Primers were always selected according to the following parameters: 30 bases length, Tm at 70°C, length of amplification product between 500 and 600 bp.

Primers were always chosen according to the following parameters: length between 18 and 25 bases, optimal 20-22 bases; Tm comprised between 57 and 65°C, optimal Tm 60-62°C 60-62°C of amplengthtiof product between 200 amplificationo as not to overlaproductthe 176 betweenlase A amplification product.

Its advantages include limited bias in the amplification of loci (maximally 6-fold compared to 10- to 10-fold in PCR-based methods), higher average length of amplification products (12 kb compared to 100 1000 bp in PCR-based methods), lower error rate (<10-6 compared to 3 × 10-4 to 10-5 for alternative methods) and higher DNA yield [ 11].

The predicted lengths of amplification products for TNF-α and β-actin were 372 and 233 base pairs, respectively.

The collecting locality, tissue source, and Field Museum of Natural History (FMNH) voucher information for each specimen, are presented in Table 1, along with GenBank accession numbers, primer sequences, and lengths of amplification products.

No significant effect of primer length on amplification success was observed.

PBS values can be used with gene length, ease of amplification and sequencing to select a suite of genes for phylogenetic inference, especially given that different suites of genes may provide more information as a function of the tempo of evolution.

This work started with that purpose: to design primers to reduce the length of D7S820 amplification products.

These individuals showed a gel electrophoresis pattern with three band, one of the same length of full amplification product and two resulting from the digestion ITS amplicon.

PCR primers for the loci DXS7132 and DXS7133 were redesigned in order to reduce the length of the amplification products compared with conventional design so that improved typing success rate when highly degraded DNA is used as a template.