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The DNA was separated in a 2% agarose gel according to a DNA base pair (bp) length marker and visualized by UV illumination after ethidium bromide staining.
DOI: http://dx.doi.org/10.7554/eLife.10319.009 To finally confirm the presence of both bound conformations in wild-type Coh Doc complexes, we replaced xylanase with sfGFPand CBM with iLOV as the contour length marker or fingerprint domains.
A recently developed alternative [ 12, 13] to full phylogenetic analysis is to infer the evolutionary relationships between known species a priori using the full length marker gene and place the shorter sequences from our samples into that reference tree.
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The obtained candidate markers were validated in independent samples covering a wide age range using alternative technologies together with previously proposed DNA methylation, sjTREC, and telomere length markers.
Note that the mobility of the studied quadruplexes is slower than would correspond to their length markers.
The correlation between G1 length and average Cln3 fluorescence intensity with different G1 length markers: WHI5-tdTomato (YCT2003) and MCM-mCherry (YCT2010).
Thus the TLC was inserted for its whole length, whereas the SLC was inserted according to length markers on the catheter.
The following DNA preparations were used as DNA fragment length markers: 1 kbp DNA ladder and pUC19/MspI DNA ladder (SibEnzyme Ltd., Russia).
Both the Low Range GeneRuler DNA Ladder (Thermo Scientific, Lithuania) and 10bp DNA ladder (Invitrogen, Carlsbad, CA, USA) were loaded as length markers.
The mRNA contained a single phenylalanine codon followed by three clusters of three sequential phenylalanine codons as chain length markers at positions 5, 25 27, 55 57, and 85 87.
Products of the PCR reactions were visualized with ethidium bromide on 1.2% agarose gels with GeneRuler 100 bp Plus or 1kb Plus DNA Ladders (Thermo Fisher Scientific, formerly Fermentas, Lithuania) as length markers.
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