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The use of a variable length gap between blocks allowed us to detect a significant motif that contained one of the recognition elements of the soxbox in all three clusters.
For wildcards, this consists of single wildcard equivalency "01.W", where W is the maximum wildcard length allowed; e.g. for the default maximum wildcard length of 2, the wildcard equivalencies are 0, 1 and 2, and a variable length gap of 1 or 2 is represented by the equivalency [12].
A 32,101 bp length gap region from AC189045 was later excluded because the predicted genes were phage related and it was decided the sequence data were contaminated.
The set S/ P stands for all the substrings of S that match pattern P. The notation x(n, m), 0 ≤ n < m, is used for a wildcard region with minimum length gap of n and maximum length gap of m, and x(n) stands for a rigid-length n gap.
The only region found with no overlapping contigs was tested by alignment with the 454 chloroplast genome and Sanger sequencing and it was confirmed that the contigs were correctly assembled adjacent to one another against the Citrus sinensis scaffold, but with a zero length gap in the T. ciliata chloroplast consensus sequence.
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It should be noted that the full length GAP-43 protein localizes to presynaptic growth cones in vivo.
A structured motif allows variable length gaps between several components, where each component is a simple motif, which allows either no gaps or only fixed length gaps.
The algorithm searches for motifs expressed as simplified 'regular expressions' (consensus sequences allowing wildcards but not variable length gaps).
We look at the problem of mining structured motifs, which allow variable length gaps between simple motif components.
For each alignment, identity was calculated as the number of matches over the selected length (gaps do not count).
Ultimately, Tcas5.2 had a higher N50 than the automated Stitch output, 4.46 Mb (Table 2), with 66 gaps with positive estimated lengths and 24 negative length gaps.
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