Exact(4)
The full length EST sequence is 97% identical with the HPV 16 E5 gene and was likely amplified from HPV sequences in the originating uterine tumour lesion.
The full length EST sequence is 97% identical with the HPV 16 genome, more specifically the E5 gene, and therefore was likely amplified from HPV sequences in the original lesion.
This was accomplished for each full length EST sequence using Phred software [ 44] to eliminate low quality regions with more than one N in a 100 bases or a stretch of bases with Phred quality score < 20.
The transcripts were selected from the chicken genome draft available in December 2004 and extensive matching of the UMIST and DT40 full length EST's with the TIGR clusters (http://chick.umist.ac.uk/).ac.uk/
Similar(56)
To reduce the inherent redundancy and increase transcript length ESTs are routinely assembled into longer consensus sequences, also known as contigs.
This is important for assembling a maximum number of full length ESTs and for avoiding chimeric assemblies of homeologous and paralogous gene copies.
Improvement in the functional predictions for 3082 mapped genes was attained by aligning partial length ESTs (File S5) to longer P. taeda transcript assemblies.
ESTs were screened for the presence of polyA- or polyT-tails (which, if present, were deleted) and re-evaluated for length; ESTs with fewer than 100 bases were removed.
Most of the hits represent different length ESTs corresponding to one of the three previously cloned LMPP precursor genes (LMPP 1 3) [ 19, 20], which again shows the high abundance of these pacifastins in locusts.
All of the genes belonging to these groups are expressed (as full length ESTs), but some lack key catalytic residues, indicating they probably encode catalytically inactive proteins with possibly novel non-peptidase roles.
In these cases, an assessment of optimal assembly parameters is needed to generate full length ESTs and avoid the production of chimeric sequences formed between homeologous copies, recently duplicated, and very similar genes.
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