The presence of full-length complements of viral genomic RNA is a hallmark of RNA virus replication within an infected cell.
Because imbalances in the synthesis of genomic RNAs and their full-length complements are common during RNA virus infections, false priming of the more abundant strand during the RT step of the assay is likely to inhibit accurate quantification of the less abundant strand, when standard unmodified virus-specific primers are used for reverse transcription and qPCR.
These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced.
As expected, over-expression of full length Rad60 complements the ts and DNA damage sensitivities of rad60-1 (Figure 3B).
On a less grandiose scale, we remove a tree, we put in a pool, we insist on a certain variety of grass and we further insist that it be mowed to uniform length and complemented by plants and flowers that a paid expert has picked out and arranged until, finally, our little square of nature is as tame and picturesque as a page in a magazine.
The castle is located on a small plateau 226 m above sea level, and comprises a central hexagonal keep, built around an inner courtyard and containing two-storeyed halls along its entire length, and complemented by an outer wall enclosing an outer yard on its western side.
The CCM1 ORF lacking PPR2, which is the most canonical motif in the full length Ccm1p, complemented neither B [ 14] nor I Δ ccm1 segregants (J. I. Moreno and M.A. Piva, unpublished work).
The ingredients are all cut in harmony, the chicken in small cubes and the spring onions in short lengths to complement the peanuts.
As shown in Fig. 4b, ectopically integrated full-length Smmob3 (pMob3FL) complemented the sterile phenotype of ∆Smmob3.
The increased chain lengths enhanced complement-mediated killing by neutrophils in vivo, and longer-chain forms were out-competed by the wild type strain in a mouse model of systemic infection.
MASiVEdb offers, through multiple and often novel ways, a comprehensive, highly curated and detailed report on the full-length Sirevirus complement of each species, while it additionally includes an integrated system for analyzing user-provided sequences against MASiVEdb elements.
