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(see supporting information on the method of calculating DNA length and coverage, supplementary material 1).
The length and coverage of rods deposited using thiocarbamide additive is almost two times higher revealing the higher growth rate.
Using these data, the fraction of repeats of each length left unresolved, given a particular read length and coverage depth is then determined.
In this respect, such an approach would constitute a major improvement in the collection of high quality data, if compared to available studies based on interviews where the participation rate was much lower and where logistics and resources limited the length and coverage of the survey [9], [30].
Moreover, the performance of PASSion is not affected by read length and coverage.
Genome length and coverage were estimated following Echt et al. (2011).
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For the simulation, reads from 503 non-overlapping gene models on human chromosome 20 were generated at varying read lengths and coverage levels.
The simulation dataset, generated from human chromosome 20, provides a measurement of the number of junctions detected and the false positive rate at different read lengths and coverage levels.
The average read lengths and coverage values from each sequencing platform are summarized (Table 1).
Seed lengths and coverage threshold are set automatically, given the genome length and the average error rate.
We observe that PHLAT outcompetes most of the existing methods over a wide range of read lengths and coverage.
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