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Note that Additional file 5 contains Tables S1, S2, S3, S4A, B, C, D and the legends of Figures S1, S2, S3A, B, S4, S5, S6.
Note that legends of Figures 3B and 3D preserve the ordering by log-P-value difference.
Enzymatic determination or quantitative RT-PCR measurement were repeated independently at least 3 times as stated in the legends of figures and tables.
In experiments exposing G. lamblia vegetative trophozoites to actinomycin D, trophozoites were cultured in medium 45 µg/ml actinomycin D (in PBS) for indicated time in the legends of Figures 4B and C. Total RNA was extracted from G. lamblia clones C6 at the indicated differentiation stages in the legends of Figures 1, and 3 7 using TRIzol reagent (Invitrogen).
Details about scenarios are given in the legends of Figures.
The detailed protocols of experiments were presented in legends of Figures 1– 6.
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We have updated these in the figure legends of Figure 4A.
For example legends of Figure 7 and Figure 8 differ only in one word.
To emphasize the symbolic nature of the actin representations we modified the figure legends of Figure 6A and Figure 6F.
Legend of Figures 8, 9 and 10: It is claimed that 'every dot represents one gene'.
A summary of major steps of the protocol can be found in the legend of Figures S2 A and S2B.
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