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This suggests that Lbe and Vg are not the only players that diversify leg disc myoblasts.
However, the mechanisms governing the myogenic programme in the developing leg disc remain largely unknown.
We therefore wondered whether Wg regulated Lbe in the leg disc myoblasts.
Subdivision of leg disc associated myoblasts into Vg-positive proximal population and the Lbe-expressing myoblasts of the leg disc proper suggests that Vg and Lbe repress each other.
Our data demonstrate that Lbe is expressed exclusively in myoblasts that populate the leg disc proper and is excluded from the myoblasts underlaying proximal portion of the leg disc epithelium that contribute to the ventral thorax.
Interestingly, the Lbe-negative proximal leg disc myoblasts express vg, previously found to control IFM development [25].
Similar(16)
Another example for genes involved in adult muscle diversification is ladybird, which is widely expressed in leg disc-associated myoblasts and required for normal leg muscle development [27], [28].
Leg discs and salivary glands were stained as described in the online methods.
Likewise in leg discs ectopically expressing Vg, Lbe was inhibited leading to an adversely affected leg muscle pattern Fig. S2.
Ectopic Dpp signaling in leg discs leads to reduced wg ligand expression and phenotypes indicative of loss of Wg signaling.
Likewise, lms is also restricted to a small area of adepithelial cells in third instar leg discs (Fig. 6B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com