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The mixture was left to react with intermittent manual stirring for 75 90 min. Finally, the solvent was removed by filtration, and the resin was washed by using DMF (10×1 min) and DCM (10×1 min).
This solution was left to react with 100 μl of BSTFA (N,O- bis- trimethylsilyl -trifluoroacetamide) at 60°C for 1 hour [ 33].
The antibody for Fhit protein (IBL, Gunma, Japan, 1 : 20) was left to react with the sections overnight at 4°C.
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The solution was left to react for two hours and was afterwards titrated with 0.1 M sodium thiosulfate.
The solution was left to react for another 3 h.
The reaction is left to react at room temperature.
Each tested compound (0.01 600.0 μM) was diluted (100X) with the ABTS+ solution to a total volume of 1 mL and then left to react for 10 min.
Chelators were added to whole blood (WB) and left to react for 3 minutes.
Desferal was added to WB and left to react for 3 minutes.
This solution was sonicated for 1 h and left to react for 18 h.
When plants evolved, for example, they began storing huge amounts of carbon in wood and other tough tissues, leaving less to react with oxygen and pull it out of the atmosphere.
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