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Crosses were performed in laying cages with apple juice agar plates and flies left to lay overnight at 22°C with embryos collected the following day, with the exception of the Src42ADN and draper RNAi overexpression experiments, in which embryos were laid at 29°C overnight.
At this time, the 3 females from the replicate crosses were placed together into a small egg laying chamber outfitted with a disc of coloured media [ 106], and left to lay eggs for an additional 24 hours, as the effects of males on egg size may not be detectable until 24 hours after mating occurs [ 25].
Females were subsequently set up in oviposition jars and left to lay eggs for two days before being released.
w; AGFP[23]/TM6B flies crossed to Oregon-R flies and only Oregon-R flies were left to lay eggs for 12 hours in regular food-containing vials.
An. funestus larvae are generally difficult to rear and therefore collection of field mosquitoes mostly relies on collecting blood-fed or gravid indoor resting females that are left to lay eggs.
They were transferred to cages and left to lay eggs, which were transported to the Liverpool School of Tropical Medicine (LSTM), UK.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com