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Cells were left to attach for 4 6 hours before treatment.
In 24-well culture plates, 2.5 × 105 cells were seeded in complete medium and left to attach overnight.
Cells were left to attach for 4 6 hours and treated with gold nanoparticles for 24 hours.
For internalisation, PC-3 cells at confluence were placed in six-well plates and left to attach overnight.
Cells were seeded into 12 well plates at a density of 1 × 105 cells per well and left to attach for 4 6 hours before treatment.
Since there are many RLuc8 molecules on the QD surface which cover the majority of the QD surface area, it remains to be tested whether there will be enough space left to attach enough ligands for desirable targeting efficacy.
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Samples of cardiomyocytes were added to the recording chamber and left to settle and attach to the bottom.
Cells were plated in 100 μL of medium and left overnight to attach.
This leaves room to attach a "header expander" to the top of the new window.
Cells were left 2 3 h to attach.
At the extreme, John Cage supplies "materials" (lines, dots, shapes) and leaves the performer to attach musical significance to them.
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