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The cells then left to adhere for 24 h in incubator at 37°C 5% CO2 level.
The 231-H2N cells (2 × 105) were seeded in 96-well plates and left to adhere overnight.
In particular, HT-1080 cells were plated in black flat-bottomed 96-well microplates at a density of 10,000 cells/well, and left to adhere overnight.
The lymphocytes were washed in fresh media before being left to adhere for 2 h in a 37°C incubator.
Fibrils were deposited onto a freshly cleaved piece of mica at a concentration of 15 µM and left to adhere for 60 min. Samples were then washed with distilled water and blow-dried under a flow of nitrogen.
Each preparation was added at a final concentration of 100 µg/ml to MDA-MB-231 breast cancer cell suspension before plating the cells, which were left to adhere to platelet-coated surfaces for 1 h.
For survival assays, the cells were seeded in complete media and left to adhere for 16 hours followed by a 3 hours starvation in serum-free medium before addition of 10 µM cilengitide or EMD135981 for another 2 hours.
Cells were plated in a droplet of medium on poly-L-lysine (100 µg/ml, Sigma, Germany) coated coverslips and left to adhere for 30 minutes before the coverslip was flooded.
Cells were left to adhere to plastic for 4 hours.
The cells were left to adhere to the inserts overnight.
Briefly, 10 cells/well were seeded in a 96-well plate and left to adhere overnight.
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