To test this model, we specifically disrupted astral microtubules by treating embryos with a low concentration of nocodazole (Noc) for 30 min, which inhibited astral microtubules but left the spindle intact.
g Photograph of CFs wound from the spindle (left) onto a second spindle (right).
(E ) Close-up of model in A showing germ line stem cells (spermatogonia, magenta) can undergo either self-renewing (parallel spindle angle, left) or differentiating divisions (perpendicular spindle angle, right).
Differences in sleep spindles were evaluated by four-factor Analyses of Variances (ANOVAs) for repeated measures with the within-subject factors spindle TYPE (fast spindles, slow spindles), HEMISPHERE (left, right), FRONT/CENT (frontal electrodes [F3, F4], central electrodes [C3, C4]) and the between-subject factor ENHANCEMENT (memory enhancers, memory non-enhancers).
(C ) Graphs show the quantification of second division monopolar spindles (left) and embryonic viability (right) after sas-4 RNAi) sas-4 RNAi with either andT orescue sas-4::gfp transgene.
At different times in the example spindle shown in Figure 4, maximal activity is seen in the left parietal, left orbital, left occipitotemporal, right occipital, right temporal, and right parietal.
The inner portion of the central spindle was left intact.
Integrated intensity profiles for (top) Plk1 and (bottom) Aurora A at the mother spindle pole (left).
Integrated intensity profiles for (top) cenexin and (bottom) p-Gravin at the mother spindle pole (left).
Even when the oocyte's original spindles were left in, the defect remained--suggesting that lack of the original spindles may not be the only problem. .
