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Left lungs were transplanted and reperfused (warm ischemia 98 ± 14 min).
It shows diffuse hilar haziness with increased density at the base of the right and left lungs.
Patches were placed on the dorsal skin over the right and left lungs to generate bilateral lung damage.
The histology scores were similar in all groups with no detectable differences between the right and left lungs.
To determine whether the new organs worked, the researchers removed rats' left lungs and stitched in lab-grown replacements.
Left lungs were homogenized in 1 ml 0.9% normal saline.
For cytokine and chemokine measurements, left lungs were excised, weighted and homogenized in saline four volumes of saline.
Left lungs were harvested and homogenized in 4 volumes of sterile saline with a tissue homogenizer (ProScience, Oxford, CT, USA).
Right and left lungs were rinsed with cold PBS and rapidly frozen in liquid nitrogen and stored at −80°C for subsequent measurement of thiobarbituric acid-reactive substances.
Bronchoalveolar lavage (BAL) was performed by advancing a flexible bronchoscope into subsegmental bronchi in the right and left lungs at both UW and IU.
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For example, to remember right versus left-sided CHF, think "Left" = "Lungs".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com