Replications (n = 4 in 2007 and n = 5 in 2008) consisted of 5 pairs of newly emerged adults each, placed in 1.3 L volume plastic cylinders with tansy ragwort leaves inserted into a moist horticultural sponge.
A 1077-bp fragment of pie-1 left homology was inserted into the XbaI site before the rpl-28 promoter and a 1017-bp fragment of pie-1 right homology was inserted into the SalI– ApaI site after the rpl-28 3′-UTR.
Sealed aluminum capsules containing tea leaves are inserted into the machine, which then recognizes which of the 25 blends — including black tea, green tea, white tea, blue tea and infusions — has been selected.
For detection of H2O2, the leaves were inserted into 1 mg ml-1 fresh DAB solution (pH 3.8) prepared in 10 mM phosphate buffer (pH 7.8) and incubated in light until brown spots were observed.
In each vessel, four cuttings with 3-4 internodes and 1-2 apicaleaveses were inserted into the mesh so that the roots could form between the wall of the vessel and the glass slide (slotted 1 cm away of the vessel wall).
Australian trade unions have been pushing for paid domestic violence leave to be inserted into government-mandated minimum working conditions for all workers nationwide for some time.
Contractile function was assessed by measuring left ventricular developed pressure (LV/dP), using a saline-filled latex balloon (0.01 mm, diameter) inserted into the left ventricle via the left atrium.
The left ventricular pressure was monitored continuously with a handmade water-filled vinyl balloon, which was inserted into the left ventricle via the left atrial appendage and connected to pressure transducer as described previously [ 26].
In this system, a high-density microalgal paste was attached to a supporting structure, which consisted of a glass plate, a filter paper and a cellulose acetate/cellulose nitrate membrane, to form an artificial 'leaf'; several pieces of these leaves are vertically inserted into a glass chamber (Liu et al. 2013; Cheng et al. 2013, 2014).
A polyethylene catheter was inserted into the left ventricle via the carotid artery and left in place throughout the experiment.
