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The resultant mixture is then turned with a fork and left for a further 30 days, before being mixed with water and sprayed on the tea bushes.
The mixture on the mica was quenched with 0.05 M Tris-HCl pH 2.5 to stop the ensilication process and left for a further 5 minutes before being rinsed with 0.05 M Tris-HCl pH 7 to remove any un-bound material.
The cells were then left for a further 4 hours at 37°C before staining for CD8 and IFN-γ IFN-γescribed asove).
One µl was injected over a course of 2 min and the needle was left for a further 2 min before being slowly withdrawn.
To do this, 4 µl of a 10% late stage infected brain extract was added per well of newly passaged cells, and the cultures were left for a further 4 days to reach confluence.
Experimental and control media plates were left for a further 7 days and then analysed for proliferation.
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Leave for a further four minutes.
Leave for a further 10 minutes without removing the lid.
Turn off the heat and leave for a further 15 minutes.
If not, add more juniper berries and leave for a further 12 hours.
Top up with the juniper-infused vodka and leave for a further 36 hours.
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