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To assign a score for the skeletal muscle system, physicians were asked to rate patients' muscle strength in five different areas of the body (neck flexors, as well as upper and lower proximal extremities, right and left) by using the British Medical Research Council scale.
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The Second Amendment states, "... the right of the people to keep and bear arms shall not be infringed," but what's left out by using the ellipsis at the beginning of that quote?
Total DNA was extracted from leaves by using the CTAB method (Murray and Thompson 1980).
The optimal extraction conditions were selected based on the condition of achieving the highest total phenolics and flavonoids content in Curcuma zedoaria leaves by using the desirability function approach in design expert software.
For transient expression of YFP-AtBS14b and YFP-AtBS14a fusion proteins, AtBS14a and AtBS14b ORFs were cloned into pEarly-GW 104 destination plasmid (Bleckmann et al. [2010]), followed by transient expression in N. benthamiana leaves by using the Agrobacterium-mediated transient expression method (Kim et al. [2009]).
was extracted from the young leaves by using the improved CTAB method.
Rye genomic DNA was extracted from young leaves by using the CTAB method [ 63].
Total RNA was isolated from grapevine plant leaves by using the RNeasy plant mini kit (Qiagen, Germany) according to the manufacturer's instructions and quantified using a Nanodrop ND-1000 specThermo Scientificermo Scientific, Waltham, MA).
We constructed a full-length enriched cDNA library (PnFL2) from P. nigra buds, roots, twigs, and stress-treated leaves by using the biotinylated CAP-trapper method, together with trehalose-thermoactivated reverse transcriptase [ 25].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com