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24 h post-transfection, cells were either left adhered or detached by mild trypsinization and put into suspension on 1% agar coated tissue culture plates with 0.5% methylcellulose for an additional 24 or 48 hours, and analyzed by flow cytometry as described [19].
Similar(59)
However, several particles were left adhering to the surface of the lattice, thereby partly or entirely obstructing the cells.
Cells were left adhere for 12 hours and then compounds were added.
Moving the meniscus aligns the RCA products in the direction of the flow, and the DNA is left strongly adhered to the surface.
It leaves behind adhered particle fragments, pits and cavities despite superior excellent engineering properties.
The cells then left to adhere for 24 h in incubator at 37°C 5% CO2 level.
The 231-H2N cells (2 × 105) were seeded in 96-well plates and left to adhere overnight.
In particular, HT-1080 cells were plated in black flat-bottomed 96-well microplates at a density of 10,000 cells/well, and left to adhere overnight.
The lymphocytes were washed in fresh media before being left to adhere for 2 h in a 37°C incubator.
Fibrils were deposited onto a freshly cleaved piece of mica at a concentration of 15 µM and left to adhere for 60 min. Samples were then washed with distilled water and blow-dried under a flow of nitrogen.
Each preparation was added at a final concentration of 100 µg/ml to MDA-MB-231 breast cancer cell suspension before plating the cells, which were left to adhere to platelet-coated surfaces for 1 h.
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