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Small pieces from the middle region of the leaves were fixed in FAA (formalin/ glacial acetic acid/ 50% ethanol, V/ V/ V, 5/ 5/ 90) for at least 24 h, then dehydrated by gradient ethanol, cleared in xylene, and then embedded in paraffin for sectioning.
Pieces of infiltrated leaves were fixed in 4% paraformaldehyde with 0.1% Triton X-100 and permeabilized in Driselase (Sigma) and IGEPAL CA-630 (Sigma) plus 10% DMSO.
After staining leaves were fixed following [ 75].
Leaves were fixed in Farmers Fluid until completely cleared.
For immunocytochemistry leaves were fixed in 3% paraformaldehyde and 0.25% glutaraldehyde in phosphate buffer (pH 7.1).
Leaves were fixed in ethanol/acetic acid (3/1) for one hour, and then washed three times with pure water.
Similar(40)
This fixes the configurations of all nodes of depth 2 at t = 2 irrespective of their configurations at t = 1, and so on, until at t = 4, the configuration of the maximum depth leaves are fixed irrespective of the configuration they held earlier.
The A-leaves were fixed at 20 cm.
Left lungs were fixed in 10% buffered formalin (Baker) overnight at room temperature.
Unlavaged left lungs were fixed in 4% paraformaldehyde for histological analysis.
Left hemimaxillae were fixed in 4% paraformaldehyde (PFA) at room temperature overnight for histology.
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