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When exogenous substrate quinolinate was infiltrated into Arabidopsis leaves to increase the natural content in downstream metabolites, a clear correlation between intermediates of NAD biosynthesis was seen, showing the accuracy of our method for quantification in biological samples.
The multi-layered foliage of Q. suber seedlings allowed i) FSLE leaves to obtain the maximum photosynthetic yield for short sunflecks, and ii) SS leaves to increase their contribution to the photosynthesis of the whole plant for long sunflecks, thus, optimizing the use of light by FSLE and SS leaves during short and long sunflecks respectively.
We also selectively picked young, actively growing leaves to increase RNA yield.
Low light levels have been shown to increase allocation to above ground organs, specifically to leaves to increase production when carbon is limiting and to branches and stems to fuel height growth through gaps in the canopy [26,42,47].
These data suggest that the strong stem sink in Wray might upregulate both carbon assimilation and the phloem loading of sucrose within the leaves to increase sucrose delivery to the stem internodes.
At pre-bloom (7 June 2012, seven days before flowering), vines were partially defoliated by removing the first six main basal leaves to increase and homogenize the exposure of fruits to solar radiation.
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