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(A ) Left, Full length of PhLP1 but not its N terminally truncated mutant ΔNT-PhLP1 increases the expression level of Gαolfβ2γ7 subunits upon overexpression in HEK293 cells.
For transient expression in N. benthamiana and grapevine leaves, full-length AtNPR1, VvNPR1.1 and VvNPR1.2 cDNAs were cloned between the CaMV 35S promoter and the 35S terminator sequences of the pUCAP-intron vector [ 42].
Among other truncations, NEP generates N-terminal truncated Aβ peptides by cleavage between Arg-2 and Glu-3 or between Glu-3 and Phe-4 but leaves full-length APP unaffected [ 35, 47].
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In addition, 33 and 22 genes were down-regulated in leaves expressing both full length and truncated AC2 protein at one and two dpi respectively.
Interestingly, we observed that 41 and 29 genes were up-regulated in Arabidopsis leaves expressing both full length and truncated AC2 protein at one dpi and two dpi respectively.
For an extra-long mouse tail, leave the leg the full length.
Total RNA (2 μg) from abdomens of newly emerged adult males was dephosphorylated with calf intestinal phosphatase, followed by removal of the 5' mRNA cap structure using tobacco acid pyrophosphatase, which leaves a 5' phosphate only in full length mRNAs.
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