Total RNA was extracted from seedling roots, young stems, young leaves, flag leaves and young panicles using an RNA Prep Pure Plant kit (Tiangen Co., Beijing, China).
In this paper, we assess growth dynamics of the whole plant (panicles, non-flag leaves, flag leaves, stems/sheaths and roots) over the reproductive development of Oryza sativa.
Iron partition quotient in panicles, non-flag leaves, flag leaves and stems/sheaths during grain filling (GF) and full maturity (FM) stages of rice plants cultivated with 5, 20 or 200 μM of Fe(III -HEDTA.
Contents of Fe, Zn, Cu, Mn, Mo, Ni, Ca, Mg, K and S in panicles, non-flag leaves, flag leaves, stems/sheaths and roots collected from rice plants supplied with different Fe concentrations at two reproductive growth stages.
Mn, Mo and Ni concentrations in panicles, non-flag leaves, flag leaves, stems/sheaths and roots collected during grain filling (GF) and full maturity (FM) stages of rice plants cultivated with 5, 20 or 200 μM of Fe(III -HEDTA.
Rice organs (panicles, non-flag leaves, flag leaves, stems/sheaths and roots) were collected at panicle exertion, grain filling and full maturity (R3, R5 and R9 stages, respectively, according to Counce et al.2000).2000
To understand the tissue expression pattern of TCM5, we extracted wild type RNA from young roots, young stems, young leaves, flag-leaves and young panicles with TRIzol Reagent (Invitrogen; http://www.invitrogen.com) and DNase I treated RNA using an RNeasy kit (Qiagen; http://www.qiagen.com) following the manufacturer's instructions.
Branches that are perpendicular to the prevailing winds are often killed, leaving "flag trees" that point in the direction of the wind.
For Zn, low Fe supply resulted in the lowest Zn PQ values in panicles, non-flag leaves and flag leaves, and high Fe supply resulted in the highest Zn PQ values (Figure3b).
From left: flags of the Ukrainian marines, Ukrainian national flag and the Russian national flag wave at the the Ukrainian marine base in the city of Feodosia, Crimea.
They leave flags, flowers, kiwi fruit, even a pair of red socks, which Blake had inspired a country to wear in 1995 during the Cup.
