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At the site in Maryland, leaves express a more modest δ13C range (∼6‰), with a clear trend that follows both light and leaf height.
N. attenuata leaves express two major plastidial lipoxygenases, lipoxygenase 2 (NandX2) and lipoxygenase 3 (NaLOX3), involved in the supply of hydroperoxy-fatty acids for green leaf volatiles and JA biosynthesis, respectively [ 28, 29].
These low values are within the false discovery range in assigning expressed transcripts, and we conclude that shielded ears and leaves express distinctive gene suites after CLI. Figure 5a shows 628 transcripts that are changed by at least 2-fold (p < 0.05) in irradiated leaves after 1 h of UV-B compared to non-irradiated plants, increasing to 1073 transcripts after 2 h.
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For the time-study of leaves expressing AtWRI1, the leaf area with GFP expression was instead divided into three; one for starch analysis, one for RNA extraction, and one for lipid analysis.
Real time quantitative PCR was used to study the expression of VvPR1 and VvSTS in grapevine leaves expressing AtNPR1 and VvNPR1.1, 3 days after agroinfiltration.
Fold-changes of gene expression given here are therefore the averages from leaves expressing the different WRI1 homologs.
Differential gene expression is given as log2 of ratios of RPKM in leaves expressing the WRI1 homologs as compared to transformed control (log2 ratio of +3 means that a transcript is 2 = 8 times higher expressed).
Therefore, it was not surprising that leaves expressing WRI1 homologs in our study indeed had a much higher expression of genes involved in glycolysis and FA synthesis pathways, as compared to in transformed control (Fig. 5, Additional file 6).
MC increased the partitioning to leaves, expressed as leaf/shoot ratio.
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