Because of the small size of leaves of the needle-leaved species, gas exchange was measured on several leaves enclosed simultaneously and manoeuvered to occupy most or the entire chamber window, without overlapping.
At FTSW 0.2, the two last ligulated leaves (youngest source leaves) and the expanding leaves (sink leaves, enclosed in the sheath of oldest leave) were sampled in the morning (before 10 am) to analyse NSC content: hexoses (glucose and fructose), sucrose and starch.
Flow cytometric analysis showed that a subpopulation positive for both GFP and sTn emerged (Fig. 1, left, enclosed by a rectangle).
Briefly, trees were infested with L. dispar larvae on one leave, enclosed with a PET bag ("Bratschlauch", Toppits, Minden, Germany) by fixing the ends of the bags with cable binders.
Leaves were enclosed in the cuvette and instantaneous gas exchange was logged following stability in cuvette conditions (after ∼30 s).
For TPS10 products in the headspace of glasshouse-grown plants (n = 4), leaves were enclosed 24 h after W + OS treatment and the headspace was collected from 24 32 h during the peak emission of sesquiterpenes in N. attenuata (Halitschke et al., 2000).
Briefly, polyethylene terephthalate (PET) containers were used to enclose leaves (300 mL sampling volume) and flowers (30 mL sampling volume) for headspace sampling.
3 Put the tins into a large plastic bin bag, which can be tucked under the tins to leave them enclosed with plenty of air.
When I left the enclosed event space, it wasn't two minutes before a man with a megaphone pointed directly at me and called me a filthy dog headed for hell.
It has a mass of small, single white flowers and small leaves that never really enclose the plant like a carapace.
