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Finally, the RNA yield and the abundance of cellular transcripts decreased as the leaves approached total PCD, as has been shown previously by others [ 53].
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For our application it is important to preserve size and separation of features such as nuclei, so we typically use the dead leaves approach [ 7], which simply replaces overlapping regions with the new frame.
My heart grew lighter and lighter as the time to leave approached.
When I was expecting my first child, I panicked when my maternity leave approached.
Although individual frame boundaries can become apparent when using the dead leaves approach, spatial features within the image maintain their size.
They stopped talking abruptly, and two got up immediately to leave, when approached by the Guardian.
As the family left, I approached the man and asked him for his thoughts on the recent raid.
Two young adults packed up and left once approached.
When I was about to leave he approached and asked if he could show me something.
The man didn't leave and approached the employee five minutes later, asking, "Do you remember me?
After he left, Goodall approached the mound and repeated the behaviour because she was unsure what the chimpanzee was doing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com