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BLAST algorithm parameters were set for "somewhat similar sequences" (BlastN), with other parameters left at default settings.
Next, as a means of minimizing alignment errors, all abnormally aligned read pairs were realigned to the reference genome with novoalign (version 2.07.11; http://www.novocraft.com, last accessed December 27 , 2013 using a k-mer size of 14 (k = 14) and step size of 1 (s = 1), with all other parameters left at default settings.
The Internet section can usually be left at default settings, unless you received specific instructions from your internet service provider.
If Sonicwall was left at default settings, you may be able to access the site you want with this method.
We set the transition/transversion ratio at 2.0; other parameters were left at their default settings.
All other options were left at their default settings.
Blastn parameters for match/mismatch score, gap open and gap extension cost were left at their default settings.
All parameters were left at their default settings, with the following exceptions: maximum trees in memory = 10000, and collapsing rules = 0 max.
All other parameters were left at their default settings.
All other priors were left at their default settings.
For assignment of ambiguous NOESY cross-peaks with ARIA, the frequency window sizes were left at the default settings of 0.02 p.p.m. for direct and 0.04 p.p.m. for indirect proton dimensions, and 0.5 p.p.m. for heteronuclear dimensions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com