For the two least stringent criteria (no cutoff or ≥10 small RNAs mapping to a gene), we observed no significant difference in the microarray expression value for the three categories of protein coding genes (Additional file 1: Figure S5A and Additional file 1: Figure S5B).
For all data sets, the number of alignment positions was reduced with gblocks applying least stringent selection criteria.
To assess the validity of our predictions, we selected QTL2 (on chromosome X) for experimental validation as this QTL, despite being important in both the 16% and 17% pool seemed to be one of the more difficult QTLs to detect (only confirmed by the least stringent selection criteria).
Regardless of these potential caveats, our results indicate that reliable transcriptional analysis of tumour cells isolated with the DEPArray is at least feasible if stringent criteria for RNA quality are applied.
Plainly, the doctrine presupposes a stringent criterion for knowledge, at least as demanding as the test for techne.
We used a stringent criterion (each site with at least three ESTs/reads support) and new annotation file (v4.3) to filter the unique poly(A) site and obtain PACs that can appropriately define high-quality APA events.
Microarray analysis showed that 64 SP and SPH genes have transcriptional activity in at least one tissue or organ according to a stringent criterion for definition of expressed genes [ 15].
For further analyses, we retained only CNVs applying a more stringent criterion, namely CNV regions (CNVRs) containing overlapping CNVs recalled by at least two programs, spanning three or more consecutive SNPs and detected in a minimum of two animals.
Of the two assignment criteria we use, both based on K2P [ 32] genetic distance (Table 1), the least stringent is "best match".
This is a fairly stringent criterion.
