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Three different approaches have been proposed or at least analyzed.
Overall 40 samples were at least analyzed with each of the six evaluated methods.
Multiunit data should be removed from the data set, or at the very least analyzed and reported separately in text and figures.
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We filtered out weakly covered introns and kept SJs that were covered by at least 10 junction reads in at least one analyzed F1-hybrid.
Clusters 1-4 represent genes that are downregulated during at least one analyzed time point compared to the BV stage, whereas clusters 5-8 represent genes that are upregulated during at least one analyzed time point compared to the BV stage.
A major inhibitor — at least as analyzed by my dining companions and me — are the prices.
Ninety-five sites were heterozygous in at least one analyzed stock.
Splicing junction were filtered by coverage (minimal coverage of 10 reads in at least one analyzed genotype).
In order to analyze genes and isoforms, we set 0.5 FPKM (at least one analyzed condition, with/without E2 stimulus) as the expression level threshold.
The crosses were performed at least four times with at least four flies analyzed per cross.
At least three fields were analyzed comprising at least 100 cells per condition.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com