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Exact(28)
Linear leak currents were digitally subtracted by −P/4 leak subtraction.
Leak subtraction (based on a standard P/n protocol) was used to subtract the capacitive component of currents.
P/N method was used for leak subtraction.
The cells requiring any leak subtraction were omitted.
No leak subtraction was used during current recording and current densities were normalized to cell capacitances.
Statistical significance was tested with paired t-test on currents at +30 mV after leak subtraction.
Similar(29)
The non-linear hyperpolarizing current was recorded at the identical voltage steps with an online leak-subtraction protocol.
All on-cell and whole-cell voltage-clamp recordings were subject to P4 subtraction, with response amplitudes determined from the peak leak-subtracted current at each test voltage.
CRAC currents shown were leak-corrected either by subtraction of the initial trace or that following application of 10 μM La3+ at the end of the experiment.
Leak currents were subtracted.
'Gating' currents were extracted from recorded current traces by subtraction of linear leak current and linear capacitive transients.
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