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Finally, leaflets were washed three times with sterile water and transferred to agar-water petri dishes.
Then, leaflets were washed in 10 mL Isoton II (saline buffer), and the sporangia production per leaflet was determined with a Coulter Z2 counter (Beckman Coulter France, Villepinte, France).
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Then, each leaflet was washed in 10 mL saline buffer (Isoton II), and sporangia were counted with a Beckman Coulter Z2 cell counter (Beckman, Villepinte, France) to determine sporangial production per lesion.
To assess RM performance, infested leaflets were detached and mites (all stages) were washed off by rinsing the leaflets one by one for 20 seconds in 25 ml 100% ethanol.
Infested leaflets that came from the same plant were washed in the same solution.
In this experiment, we infested leaflets on intact plants with 2, 4, 8, 16, 32 and 64 mites per leaflet on each of three leaflets per plant and after 14 days the mites were washed off, as described earlier.
After 3 h, cells were washed in PBS and we used an Annexin-V-FITC to assess outer leaflet phosphatidylserine (PS) exposure in the plasma membrane as a means for the early detection of apoptotic cells.
After 3 h, cells were washed in PBS and we used an Annexin-V-FITC (Roche) to assess outer leaflet phosphatidylserine (PS) exposure in the plasma membrane as a means for the early detection of apoptotic cells.
Dishes were washed.
His hands were washed.
They were washing burnooses.
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CEO of Professional Science Editing for Scientists @ prosciediting.com