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Leaflets were then dropped into the homes of civilians in the northwest of Gaza, asking them to evacuate, ahead of what a military spokesman described as a "short and temporary" assault on the region.
Leaflets were then inoculated with 6 evenly spaced 10 µl drops per leaflet, for a total of 24 independent inoculations per isolate/genotype interaction.
Leaflets were then placed in sample boxes that had been prepared as described above.
Inoculated leaflets were then incubated as described for the virulence tests.
Leaflets were then cut off at a petiole and subjected to a series of stress treatments that included dehydration, chilling, heating and exposure to NaCl, abscisic acid, salicylic acid, jasmonate, and H2O2 for varying periods (1, 2, 5, 10, and 24 h; except, 6 h for the salicylic acid and jasmonate exposure).
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Total area of the leaflets is then reported as leaf area.
The tricuspid valve leaflet was then re-sutured to its annulus using a continuous suture (5-0 polypropylene), and the right atrium was closed.
For each residue type the abundance in the inner leaflet was then subtracted from the abundance in the outer leaflet, and divided by the total abundance.
A full invitation letter as well as participant information leaflet was then sent out by a research nurse to eligible respondents.
A patient's leaflet was then written by a medical journalist with the help of expert patients from the French Cancer League, and was made available only on a password-protected website (http://www.fnclcc.fr/doc/pdf/info_patients/prive/trait-union-no13.pdf (User=BECT; Password=PACS04)) (see Supplementary online material).
These leaflet discs were then flash-frozen in liquid nitrogen until required.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com