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For the detached leaflet assay, four leaflets from each replicate were sampled while three leaflets were sampled and pooled from each replicate for RNA extraction.
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For compound-leaved species, the measured leaflet was sampled.
Briefly, the terminal leaflet of the first fully expanded leaf was sampled from wild-type and AtGRXS17-expressing 4-week-old plants.
Leaflets were then placed in sample boxes that had been prepared as described above.
After seven days of infestation (the time-point of sampling) symptoms of RM-infestation were clearly visible, but leaflets were not necrotic or senesced.
The leaflets were relatively acellular.
Over 28,000 leaflets were distributed.
Thirty-six patinformationation leaflets were identified.
The leaflets were printed in Wales.
The leaflets were pressed and dried.
Leaflets were hand delivered to each household.
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