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After 7 10 days, sporangia or mycelia from the leaflets were harvested and transferred onto RVN media.
Young leaflets were harvested on each plant, and DNA was extracted using a standard CTAB (hexadecylatrimethylammoniumbromide) procedure [ 49].
The progress of C. truncatum infection was microscopically assessed and infection sites of leaflets were harvested as described above at 3 time points: appressorial penetration phase (16 hai), biotrophic stage (44 hai), and necrotrophic stage (68 hai).
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One inoculation spot per leaflet was harvested 24 hours post-inoculation as leaf discs with 10 mm in diameter, and frozen in liquid nitrogen.
Four leaflets per plant were harvested on ice.
At 16, 24 and 48 hours after inoculation (hai) leaflets of control and inoculated plants were harvested, immediately frozen in liquid nitrogen and stored at -80°C.
For library construction, leaflets of 3-week old lentil cultivar 'Eston' were harvested and inoculated with 15 μL droplets of conidial suspension (4 × 104 conidia mL-1) of C. truncatum isolate CT-21.
Mouse external ear pinnae were harvested and separated into dorsal and ventral leaflets.
Four-week-old plants were treated at 4 °C and the terminal leaflet of the first fully expanded leaf was sampled from wild-type and AtGRXS17-expressing plants were harvested on 0, 1, 3, 5, 7, 12, and 21 days, respectively.
Six leaf samples were harvested per individual at the 6-leaves stage and always came from the apical leaflet.
Supernatants were harvested carefully.
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