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For an estimation of trichome density, the abaxial part of three leaflets was observed under the binocular microscope and a visual scale was used to describe it.
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Membrane-spanning dimers between lipids in different membrane leaflets are observed only if the pyrene moiety is attached to the latter half of the acyl chain.
The terminal central leaflet was observed and classified as "hastate" or "sub-globose" five weeks after germination for each of the RILs.
Plants were scored 1 when less than one quarter of the leaflets were visually damaged, 2 for one quarter of the leaflets being affected, 3 when freezing damage was observed on half of the leaflets and 4 when three-quarter of the leaflets were concerned.
Severe calcification was observed in all leaflets and aortic annulus.
Severe calcification was observed in all leaflets and aortic annulus (arrowheads).
Collagen accumulation was observed in FED leaflets and chords and decreased in BD.
Another band around 1,098 cm−1 responsible for crystalline cellulose was observed in both leaflets and rachis.
Slightly modified functions of metallothioneins have been reported in plants, where their increased expression was observed in senesced leaflet and abscission zones, under ethylene induction, or as a consequence of mechanical wounding when infecting tobacco with TMV virus was obsereved [ 42- 44].
Although the layered architecture of leaflets was relatively well preserved, prominent calcification was observed in the sinus wall that had been in direct contact with the surrounding fabric.
Degradation of valvular leaflet in one of the glutaraldehyde-fixed conduits was observed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com