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The court's 1995 decision, in McIntyre v. Ohio, invalidated an Ohio law that prohibited the distribution of anonymous political leaflets, using a First Amendment analysis that has created some concern about the fate of campaign disclosure requirements in general.
Three hundred 6 mm diameter infection sites were punched from the leaflets using a cork borer during 48-72 handand microscopically assessed under a compound microscope to ensure the correct timing for the biotrophy-necrotrophy switch.
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Then 57 patients belonging to 8 different GPs were interviewed about the leaflet using a semi-standardised script.
Leaflets were injured on the lower surface (one injury per leaflet) using a 0.9 mm glass pin, and 5 μL of spore suspension was pipetted onto the wound [ 37].
She held five small protests, which included handing out leaflets and using a loudhailer – set, she said, on level two of six – before lawyers acting for Harris's federation obtained an injunction against Fergus and her community group.
Total RNA was extracted from leaflets using the RNeasy kit (Qiagen).
Agreed themes/subthemes comprised the topics for round 2. Themes/subthemes from round 1 were proposed to the panel as potential leaflet content using a combination of open and closed questions.
In the absence of a suitable evaluation tool, the patient leaflets were compared using a checklist devised by the researchers.
Genomic DNA from young leaflets was extracted using a CTAB based protocol [ 28, 29] adjusted for high throughput isolation.
Centuries later, in the American Revolution, Thomas Paine's "Common Sense" was but one of many pamphlets and leaflets used to strengthen the British-American colonists' will to fight.
The leaflets used by the bogus collectors show the breast cancer pink ribbon logo and claim to be collecting on behalf of Breakthrough.
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