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Outside, however, local party members were forlornly handing out leaflets, on which they had written, "The party is going to die".
In contrast, the number of russet mites was reduced on dual-infested leaflets compared to leaflets on which russet mites were feeding alone, albeit not significantly (28.4 ± 8.3 versus 15.4 ± 2.7 mites/leaflet) (see Additional file 2: Table S1).
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Two sites had 'never' used the leaflet, one of which provided an alternative, which was a text-only leaflet providing general advice on sling use during conservative management of proximal humeral fractures.
Huhne said: "They went out and put out leaflets or sanctioned leaflets which were personally attacking Nick [Clegg] for going back on the tuition fees pledge, even though they had actually insisted on that as part of the coalition agreement and making the coalition work".
Enclosed with the letter of invitation is an information leaflet, a reply slip on which they can indicate whether or not they are willing to consider participating and a pre-paid envelope addressed to the research team.
He said: "They've spent in excess of £100,000 over the last six months on leaflets which attack me".
The tricuspid annulus, on which the leaflet sits, is a horseshoe-shaped saddle-like structure with an elliptical configuration.
In fact, a number of participants recalled a small credit card leaflet which had the telephone number on which participants could keep in their wallet.
The UKIP leader had his own leaflet to brandish later in the debate - one which featured Mr Clegg promising a referendum on the European Union.
"It is enough now!" read one of the leaflets, which went on to promise to give "the north African street children who are roaming around" the "punishment they deserve", according to reports.
Five neonate PTM larvae were weighed together, and then placed on the leaflets, after which the containers were sealed and kept in a controlled temperature room at 22 ± 3°C with a photoperiod of 16 h light: 8 h dark.
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