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Secreted protein fraction or secretome were obtained by pooling five leaflets from each replicate.
Four detached leaflets from each of three Desiree plants were infected with P. infestans two days after treatment with either 10 mM BABA or water (control).
For the detached leaflet assay, four leaflets from each replicate were sampled while three leaflets were sampled and pooled from each replicate for RNA extraction.
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Alternatively, if only one leaflet from each bilayer is involved in the fusion process, the bilayers are said to be hemifused.
In total one leaflet from each of three plants per treatment was used for RNA analysis.
Four mechanically-inoculated leaflets, two from each side of the petiole, were collected from three plants (=three biological replicates which correspond to replicates described in Additional file 1) per treatment per variety at each time point, 4 and 10 hpi.
To measure toughness we randomly selected two leaflets from leaf # 2 on the phyllotaxis in each plant of the 15 selected (male-female) pairs in 2001.
Each year, an estimated 4bn pizza flyers, leaflets from local councils and blank envelopes promising the recipient cash prizes are already thrust through letterboxes by its postal workers.
To this effect, for each plant we collected four randomly selected leaflets from the 3rd leaf in the plant's phyllotaxis.
The bottom of a 350 ml plastic container was lined with dry filter paper (Whatman®, No. 1, 50 mm diameter) into which were placed 3-5 terminaleafletsts from the youngest, fully expanded leaves excised from plants of each replicate.
Columbia University 1968 Photos, leaflets from 1967-71, and button images.
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