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Efficient extraction of recombinant CBH from transgenic corn stover leaf was achieved in a simple process using water after minimal tissue disruption.
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Leaf area consumption was assessed through using a Win/Mac Folia leaf area meter (Regent Instruments Inc .. Leaf wounding was achieved through piercing with a sharp pair of forceps.
Biosynthesis of platinum nanoparticle pellets using O. sanctum leaf broth was achieved at 100 °C in 1 h [55].
Maximum callus induction and proliferation in leaf segments was achieved on 1/2X MSBM supplemented with 0.5 mg/L−1 BA, 0.5 mg/L−1 NAA, 0.4 % gelrite and on incubation at 20 °C.
Fractionation of the ethyl acetate leaf extract was achieved by successive column chromatography which eventually led to isolation of an active fraction, F-10.
The highest expression level up to 30%% of total soluble protein (about 1 mg/g of fresh leaf tissue) was achieved with the PVX-based expression system.
Organogenesis from leaf-disc explants was achieved on MS medium supplemented with BAP alone at 5.0 µM.
A parallel time course was achieved with leaf tissues of plants agro-infiltrated with the pBIOS738 empty vector that served as reference value for siRNA accumulation.
The UV-vis spectra recorded, implied that most rapid bioreduction was achieved using banana leaf extract as reducing agent followed by tulsi and neem leaf extracts.
Maximum solar conversion efficiency of 0.5% was achieved for the C. fruticosa leaf extract obtained under the optimum extraction conditions.
A distinct apoplasmic pH signal was achieved in ~90% (N>160) of leaf impalements.
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