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Actual leaf area was calculated as: leaf area = weight of leaf tracing × conversion factor.
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This primary-thickening meristem produces vast amounts of parenchyma to the inside, through which the leaf traces differentiate.
Leaf traces possessing mesarch primary xylem.
(e-g) Individual leaf traces in inner cortex.
Two leaf traces probably divide tangentially from the rib tip.
Individual leaf traces circular or slightly oval with two protoxylem strands.
Inner cortex ca 0.5 0.8 mm thick, homogeneous, parenchymatous, containing about ten leaf traces in a given transverse section; outer cortex ca 1.5 2.5 mm thick, homogeneous, sclerenchymatous, containing about 20 leaf traces in a given transverse section.
Two leaf traces depart successively from the tip of a stem xylem rib (Additional file 6: Figure S6).
The origin of the leaf traces is unique because the two traces were produced from a stem primary xylem rib at different stages.
Two leaf traces successively derived tangentially from tip of each primary xylem rib and once divided to produce four traces in stem cortex.
Leaf traces in the cortex of these three stems have mesarch primary xylem (two protoxylem strands, see Figures 2f and 3e, k, Additional file 2: Figure S2e).
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