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It is the ratio between the concentration of metals in contaminated soil and the concentration of metals in uncontaminated soils (Mehes-Smith et al. 2013).EF of Cu in leaf portions was in the trend 20 > 30 > 10%% while in the shoots the trend was 20 > 10 > 30%% (Fig. 1).
Three leaf portions were used, weighing 3.5, 7.5 and 15 g.
Four LAZ-containing leaf portions were arranged in each embedding mould.
However, only GLVs and 1-octen-3-ol were produced in wounded areas, while (E -β-ocimene and DMNT wE -β-ocimeneriminant for thE -β-ocimeneleandportions.
The main advantages of this technique include: a) in vivo sampling; b) reproducible sampling; c) ease of execution; d) simultaneous assays of different leaf portions, and e) preservation of plant material for further "omic" studies.
(Z -3-Hexenyl butyrate, DMNT, TMTT, (E)-β-ocimene and (E)-nerolidol were produced in the same area, close to the HW Z -3-Hexenylso in distant leaf portions.
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The leaf portion may be high in aboveground litter stock [21], [27].
This result suggests that the basal segment is functional and mechanisms of senecence avoidence are active, as expected for a young and immature leaf portion.
The right half of each leaf portion was inoculated with 3 agar plugs containing actively growing Phytophthora capsici mycelium and the left half was inoculated with sterile agar plugs as negative control.
PCA data processing was performed by using the discriminating variables identified above (GLVs, homoterpenes, mono- and sesquiterpenoids) with the aim of establishing a relationship between sampling time and leaf portion.
Compared to controls, HW treated leaves showed a significantly higher production of the GLVs (E -2-hexenal, (Z)-3-hE -2-hexenalZ)-3-hE -2-hexenalte and of 1-octen-3-ol close to the HW damaged leaf portion.
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