No remarkable differences were observed in leaf POD activities between GA3, PBZ, 6-BA treatment and CK in the two cultivars during late seasons in 2007.
Overall, higher leaf POD contents were observed for Peizataifeng as compared to Huayou86, depicting a genotypic difference for leaf POD activity.
Leaf, pod, and seed samples were collected and kept in a refrigerator (4 °C).
After NaCl treatments for 18 days, the activities of leaf POD at 100, 150 and 200 mM NaCl levels were significantly higher than at no NaCl level (Fig. 4A).
As an alternative to 50% of the recommended dose of NPK, the addition of OMF compost at a rate of 10, 20, or 30 ton ha−1 showed significant decreases (P ≤ 0.05) in the concentrations of NO3 − and Cd2+ of leaf, pod, and seed of common bean plants compared to the control treatment (100% of the recommended dose of NPK; Table 5).
There were no significant differences between the data obtained as a result of the application of OMF compost at rates of 20 and 30 ton ha−1, while both rates were more efficient in reducing the concentrations of NO3 − and Cd2+ in leaf, pod, and seed of common bean plants than a rate of 10 ton h−1.
The qRT-PCR of soybean leaf, pod, and stem tissues was performed with a 96-well plate qRT-PCR machine (7500 Real-Time PCR System; Applied Biosystems, Foster City, CA).
The treatment effects were examined by measuring disease development on leaf and pod tissue, pod destruction index, pick (discolored and misshaped seed), yield and return on investment (ROI).
This treatment inactivated transport of compounds from leaf to pods occurring via phloem (a living tissue) but not from pods to leaf, which occurs via xylem (an already dead tissue at functional maturity of plants).
OsMT1e-P ectopically expressing plants accumulate negligible Na+ as well as heavy metals Cu2+ and Zn2+ in upper leaf and pods as compared to older leaves and roots.
