To determine the location of the leading edge we used image analysis software to analyse the experimental images showing the entire colony [see Additional file 1] [ 62].
This observation is confirmed by examining the magnified image of the leading edge in Figure 5F where we again see that it is not obvious whether the low q or the high q matches the measurements.
Whoa! Kanye's ex-stylist contributed some very smart shit about how the idea of "civilisation" inherently guides us toward the feminine, and then the art director adjusted his black fedora and proclaimed that the leading edge of the image is bringing the masculine to feminine ratio into balance.
Whoa! Kanye's ex-sylist contributed some very smart shit about how the idea of "civilization" inherently guides us toward the feminine, and then the art director adjusted his black fedora and proclaimed that the leading edge of the image is bringing the masculine to feminine ratio into balance.
A pre-bleach image was captured, the leading edge actin was photobleached for 15 seconds, then images were captured every second for 60 90 seconds.
8) In Figure 3M and N, Golgi staining hard to see, orientation of leading edge hard to interpret from images shown.
We repeated the leading edge detection procedure for all experimental images at t=0, 24 and 48 hours for both initial cell densities.
Using the same image analysis procedure that was applied to the experimental images [ 7, 62], we detected the leading edge of the simulated experiment, and calculated the area enclosed by the leading edge to determine R. Figure 4 E F show two snapshots from a single realisation of the model with D = 243 μmhour−1, q = 0 and λ = 0hour−1 at t = 0 and t = 48 hours.
This paper characterizes the leading edge conditions, based upon simultaneous stereo Particle Image Velocimetry (sPIV), OH Planar Laser-Induced Fluorescence (PLIF) and OH* chemiluminescence data.
Alternatively, to quantify filopodia sprouts per vessel length NIH ImageJ software was used to count projects per 100 μm at the leading edge of the migrating vessel front (n=3 images per retina, n=3 mice per genotype).
