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Analysis of leader length and velocity can reveal the influence of particular characteristics of lightning overvoltage waveform across the insulators produced by different return-stroke current waveforms in the assessment of the lightning performance of a transmission line.
Moreover, the relative effects of 5' leader length were retained even in the presence of the LNA-AUG1 oligonucleotide.
In addition, the effects of 5' leader length observed in the plasmid transfection experiments were also seen in the RNA transfections (Figure S3B).
Mutational, rather than deletional, analysis was performed to maintain sequence length, in view of the potential for influence of translational efficiency by 5'UTR leader length.
In addition, we observed that increased leader length by itself changed the ratio of the proteins and favored initiation at AUG1.
The effect of 5' leader length is consistent with our earlier studies [4], and with other studies that have reported effects on translation efficiency and AUG codon choice (e.g. see references [12], [34]).
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However, it is not possible generally to deduce the effects of specific 5' leader lengths on translation, as different nucleotide compositions may generate different secondary structures, or contain binding sites for proteins or other nucleic acids.
In cases where these two data sources cited different leader lengths for the same gene, we favored the longer estimate.
The nucleotide position of the TSSs enabled us to perform a promoter analysis, which resulted in the prediction of binding sites for 6 sigma factors, the analysis of 5' leader lengths and the prediction of 625 operons.
The questionnaire began with questions about the respondents: age, sex, length of employment in a leading position (for leaders), length of employment in a hospital, level of education, and area of work.
The architectural parameters studied were: diameter and node number of the trunk; number, diameter and angle of insertion of the lateral branches departing from the central leader; diameter, length, node number and average angle of fruiting shoots.
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