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This was especially evident from the chemical probes, with most residues sensitive to imidazole and lead cleavage.
When comparing the lead cleavage results of the investigated L7Ae RNP complexes, the most obvious differences were observed within the D-box1 region of snoRNAs (fig. 3 A).
Notably, D′, C′, and C-boxes were completely protected from lead cleavage in all tested RNPs, indicating that in the investigated RNAs those nucleotides were involved in K-turn formation and therefore bound to L7Ae.
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Upon addition of target lead, catalytic cleavage of substrate DNA segments in the double-stranded DNAzymes results in the capture of the initiator strands via the conjugated catalytic strands on magnetic beads.
Lead acetate cleavage was performed with minor modifications (Youssef et al. 2007).
For further analysis of structural elements underlying Snord115 posttranscriptional processing, we performed lead acetate cleavage experiments.
We expected that treatment with T7E1 or Surveyor nucleases would lead to cleavage of heteroduplex molecules in two smaller molecules.
These data suggest that ephrin-B1/B2 signaling does indeed lead to cleavage of adherens junctions in the advancing wound epidermis.
Expression of pepNoxa or pepBad did not lead to cleavage of PARP in A375 cells, indicating that apoptosis was not induced by these anchored BH3 helices.
The model in Figure 2(A) describes a dynamic binding, sliding and release scheme where not every sliding event would lead to cleavage.
To investigate whether DNA breaks may lead to cleavage of CREB3L1, we examined etoposide, another chemotherapeutic drug that inhibits topoisomerase (Stähelin and von Wartburg, 1991).
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