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LC 1, as he called it, was acquired by the Art Gallery of Southern Australia for $3,000.
Dissatisfied with the "art-school pretensions" of the scroll at the head of LC 1, he took another crack at the design.
Then L c 1 k Net ( n ) ) = Lc 1 k + log exp Lc ~ rk + exp Lc 2 k + IA 2 k n 1 + exp Lc ~ rk + Lc 2 k + IA 2 k n, L c 2 k Net ( n ) ) = Lc 2 k + log exp Lc ~ rk + exp Lc 1 k + IA 1 k n 1 + exp Lc ~ rk + Lc 1 k + IA 1 k n.
Let Lc 1 Net and Lc 2 Net be the extrinsic information outputs of the network decoder, and Lc 1 Dec and Lc 2 Dec be extrinsic information outputs of the coded bits of SISO decoder 1 and SISO decoder 2. Finally, let IA 1 and IA 2 be the a priori information (on coded bits) of the network decoder.
Step 1. (Network decoding) At the n th iteration, the network decoder decodes Lc 1, Lc 2, Lc ~ r, with a priori information IA 1 n and IA 2 n to output the extrinsic information of coded bits Lc 1 Net ( n ) and Lc 2 Net ( n ).
In patients' lavage fluids, Lc 1 was more likely to be partially degraded (34 kDa).
Similar(44)
In Western countries, 15%-20%-20%HCCs are diagnosed in the absence of LC [ 1, 5, 6].
Three C-terminal deletion peptides were engineered; LC 1 425 and LC 1 418 were expressed and could be purified as soluble and stable proteins, whilst LC 1 398 was soluble, but not stable to storage.
Conversion of Cys430 to Ser abolished dimer formation of LC 1 448, but did not improve solubility.
Kinetic studies showed that LC 1 448 and LC 1 425 efficiently cleaved GST SNAP25 and the fluorescent substrate SNAPtide, while LC 1 418 catalyzed the cleavage of both the SNAP25 and the fluorescent substrate SNAPtide with a similar Km, but at a 10-fold slower kcat.
Approximately 40% of LC 1 448 was a covalent dimer due to the formation of inter-chain disulfide bond formation at Cys430.
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