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During the egg laying stage, fat synthesis in chicken liver is especially active [ 75].
We detected Pitx2 in the PMZ by the time of laying, stage X, where it remains until early streak stages.
LSJ1 started laying eggs at 0.8 ± 0.04 mm as opposed to the > 1 mm classically associated with reaching the egg laying stage of adulthood [ 11].
During the laying stage, gene expression is highly stimulated in liver to support the metabolic changes associated with the development of the reproductive organs.
DE genes are considered to be important regulatory factors of lipid synthesis and transport in liver during the laying stage of chicken.
Expression is restricted to the posterior marginal zone (PMZ) already at the time of laying (stage X); this intensifies over the subsequent stages.
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Nestboxes were regularly checked during the whole breeding season, with more frequent checks in the nest building and egg laying stages.
Differences in expressed genes were found between the juvenile and egg laying stages, including highly expressed novel genes, splice isoforms, and pathways.
Embryos from homozygous Dichaete-Dam and Dam only lines were collected from 2-7 hrs after egg laying (stages 5 11) and DNA processed for DamID according to the method of Vogel et al. [ 71].
Because of approximately 1 2 weeks of uncertainty regarding the date when eggs were laid, staging was done according to the embryological series and stages described in [30] Chicken fertilized eggs were obtained from Charles Rivers Laboratories and incubated in polysterene egg incubators (Hova-bator) at 37°C with a water tray for humidity.
The observation that all of the wild isolates lay very early stage eggs and that the QTL are associated with increased bagging does however argue that laying late stage eggs is deleterious and therefore that selection will be acting to minimize this.
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