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Kunitake and co-workers constructed alternating layers of glucose oxidase and peroxidase with layer-by-layer film adsorption on a quartz slide and subsequently demonstrated sequential activity.
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In this paper, a mediatorless amperometric glucose biosensor based on direct covalent immobilisation of monomolecular layer of glucose oxidase (GOx) on a semiconducting indium tin oxide (ITO) is demonstrated.
In this work, the anion exchange resin (AER) was modified with a layer of glucose oxidase (GOD) and poly(diallyldimethylammonium chloride) (PDDA), respectively, via layer-by-layer electrostatic self-assembling strategy.
In this biosensor, a gold tube coated with a conductive layer of glucose oxidase/Nafion/graphite was used to create an interference-free region in its diffusion layer by electrochemically oxidizing the interfering electroactive species at proper potentials.
Moreover, beads' having no layer of chitosan, glucose consumption time tends to decrease by increasing flow rate.
With beads having no layer of chitosan, glucose consumption time tends to decrease by increasing flow rate.
The parameters of the biosensor including the number of assembled HRP and GOD layer, and the concentrations of glucose were optimized.
The CSCNT/felt matrix also enabled the preparation of a glucose biosensor whose sensitivity could be tuned as a function of the number of glucose oxidase (GOx) layers deposited through a Layer-by-Layer technique with an sensitivity of 11 ± 2 μA mmol−1 L achieved at 15 poly(diallyldimethylammoniumchloride)/GOx bilayers.
The diffusivity of glucose through boundary layer surrounding the biocatalyst particle plays a major role in achieving maximum ethanol yield that is directly controlled by the structure of immobilizing matrix and stirrer speed of a bioreactor.
Interstitial fluid (ISF) in the dermis layer of skin has the best correlation of glucose concentration to blood glucose levels when compared to ISF in other skin layers [ 3, 4].
Such a change might be caused by an increase in the refractive index of the more superficial layers of the lens as a result of glucose penetration.
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